24-Hour Urine Collection Instructions (Acidified)

Required Laboratory Supplies:

• Special 24-hour urine collection container
• Two yellow-capped containers with urine transfer devices
• Acidifying additive – 10% HCl, 30 mL (Keep out of reach of children. Avoid skin and mucous membrane contact)

Urine Collection Instructions:

1. Start collecting urine, e.g., at 7:00 AM. Discard the first morning urine and note the start time.
2. Collect all subsequent urine including the next day’s 7:00 AM sample, in the special 24-hour container.
   Keep the collection container refrigerated (2–8°C) during the entire collection period.
3. Before collecting the first urine sample, pour 10 mL of the acidifying additive (10% HCl) into the container.
4. After 24 hours of collection, record the total urine volume collected.
5. Gently shake the container, then use the urine transfer devices to draw 10 mL of urine into each of the two yellow-capped containers.
6. Remove the transfer devices, tightly seal the containers, and bring them to the laboratory.

Important Notes:

• During the collection period, the patient should drink approximately 1.5–2 liters of fluids.
• If the total urine volume exceeds 1 liter, add an additional 10 mL of acidifying agent for every 1 liter of urine.